學術論文英翻中_2元/字_7485字 - 翻譯

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[必]工 作 量:7485英字(確認接案會請譯者一起核對字數)

[必]工作報酬:15000

[必]涉及語言:英譯中

[必]所屬領域:生物、醫學

[必]文件類型:論文(33頁)、期刊(4頁)

[必]截 稿 日:徵到後5日

[必]應徵期限:徵到為止

[必]聯絡方式:[email protected] ; [email protected]

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[選]試 譯 文:

(2) Inhibition of fat accumulation
a. Suppresses differentiation of 3T3-L1 adipocytes (in vitro)
Experimental design and treatment:
3T3-L1 adipocytes (5x104 cells/ml) were cul
tured in DMEM medium (high glucose)
supplemented with 10% fetal calf serum. Ce
lls differentiation was induced by medium replacement containing
insulin (1 μg/ml), dexamethasone (0.25
μM) and isobutyl-methyxanthine (0.5 mM). 2 days later, the medium
containing Coffee Bean Extract and its
functional components and insulin (1μg/ml) was introduced.
The new medium was cultured for 6 days
with replacement every alternate day. Differentiation of adipocytes
and cell triglyceride levels were dete
rmined. Cell differentiation was determined using GPDH
(glycerol 3-phosphate dehydrogenase) as biochemical marker.

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